Research

Extensive studies of the reference plant Arabidopsis have enabled deep understandings of tissues throughout development, yet a census of cell types and states throughout development are lacking. We created a single-nucleus transcriptome atlas of seed-to-seed development employing over 800,000 nuclei, encompassing a diverse set of tissues across ten developmental stages, with spatial transcriptomic validation of the dynamic seed and silique. This atlas provided a resource for the study of cell type specification throughout the continuum of development, and a reference for stimulus response and genetic perturbations at the single-cell resolution.